addendum: amplification, sequencing and cloning of iranian native bacillus subtilis alpha-amylase gene in saccharomyces cerevisiae [published in jundishapur j microbiol. 2013;6(8):e7371]

نویسندگان

fahimeh afzal-javan department of genetics, faculty of science, university of shahrekord, shahrekord, ir iran

mohsen mobini-dehkordi department of genetics, faculty of science, university of shahrekord, shahrekord, ir iran; research institute of biotechnology, university of shahrekord, shahrekord, ir iran; department of genetics, faculty of science , university of shahrekord, shahrekord, ir iran. tel/fax. +98-3814424419

behnaz saffar department of genetics, faculty of science, university of shahrekord, shahrekord, ir iran; research institute of biotechnology, university of shahrekord, shahrekord, ir iran

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منابع مشابه

Addendum: Amplification, Sequencing and Cloning of Iranian Native Bacillus subtilis Alpha-amylase Gene in Saccharomyces cerevisiae [published in Jundishapur J Microbiol. 2013;6(8):e7371]

Addendum: Amplification, Sequencing and Cloning of Iranian Native Bacillus subtilis Alpha-amylase Gene in Saccharomyces cerevisiae [published in Jundishapur J Microbiol. 2013;6(8):e7371] Fahimeh Afzal-Javan ; Mohsen Mobini-Dehkordi ; Behnaz Saffar 1,2 1Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, IR Iran 2Research Institute of Biotechnology, University of Sh...

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amplification, sequencing and cloning of iranian native bacillus subtilis alpha-amylase gene in saccharomyces cerevisiae

conclusions based on this similarity and our bioinformatics evaluations, this mentioned alpha-amylase gene can be expressed in s. cerevisiae as extracellular enzyme. results the presence of alpha-amylase gene in recombinant bacteria was certificated by colony-pcr method. after extraction of recombinant vector from e. coli, the competent s. cerevisiae cells were transformed using polyethylene gl...

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CLONING AND EXPRESSION OF HUMAN IFNα2B GENE IN SACCHAROMYCES CEREVISIAE

Interferon is a protein secreted by eucaryotic cells following stimulation by viruses, bacteria, and many other immunogenes. Recent medical studies indicate that interferons have effective role in the treatment of virus infections, immunodeficiency and certain types of cancer such as hairy cell leukaemia (HCL). The aim of the present study is to apply yeast strain for secreting human IFNα2b fol...

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Isolation, Subtype Determination, Cloning and Expression of HBsAg Gene from an Iranian Carrier in Saccharomyces cerevisiae

The Hepatitis B Surface antigen ( HBsAg) gene was isolated from an Iranian HBeAg positive carrier by PCR. The gene was cloned in pUC19 for sequencing and pYES2 for expression in Saccharomyces cerevisiae, which pNF1 and pDF3 constructs were made respectively. The sequencing data showed that the isolated HBsAg gene shared more than 90% homology with the ayw subtype. The pDF3 was transferred into ...

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Cloning and Expression of Thermostable alpha-Amylase Gene from Bacillus stearothermophilus in Bacillus stearothermophilus and Bacillus subtilis.

The structural gene for a thermostable alpha-amylase from Bacillus stearothermophilus was cloned in plasmids pTB90 and pTB53. It was expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about fivefold more alpha-amylase (20.9 U/mg of dry cells) than did the wild-type strain of B. stearothermophilus. Some properties of the...

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In vitro elaboration Mutagenesis and cloning of the PA gene in Bacillus subtilis

Background: The immune antigen of Bacillus anthracis is a protein that can attach to the surface receptor of all human cells. At the surface of cancer cells, there is a receptor that activates the uPA (Urokinase plasminogen) that do not exist in normal human cells. Objectives: The aim of this study was changing the location of the attachment of the PA gene by a dir...

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عنوان ژورنال:
jundishapur journal of microbiology

جلد ۷، شماره ۲، صفحات ۰-۰

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